Cell-free design and implementation of CRISPR guide RNA switches

Project Investigator: Matthew Lakin

Project Description: The recently-developed CRISPR system is a powerful toolbox for precisely modifying genomes and for precisely regulating gene expression. Its precision derives from its use of a guide RNA, which is an RNA molecule that directs the CRISPR protein to bind to a specific DNA sequence. This project aims to engineer CRISPR guide RNAs to function as switches that can be controlled based on detection of specific molecules, thereby enabling programmable control of the activity of the CRISPR system. This could be used to provide cell type-specific control of CRISPR system, for example, for targeted cancer therapy.

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